Fluorescent probes offer sharper view of plant tissues
Researchers at the Indian Institute of Technology Gandhinagar (IITGN) and the Regional Centre for Biotechnology, Faridabad, have developed and tested a new class of fluorescent probes that enable scientists to visualize xylem, the specialized tissue responsible for transporting water and minerals through plants. The work, published in Plant and Cell Physiolo
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- Pioneers of Fluorescence Microscopy: Fredrick W. Herschel first observed fluorescence in 1845, with Sir George G. Stokes further studying the phenomenon; Carl Zeiss and Carl Reichert later developed these observations into fluorescence microscopy in the early 1900s, and Oskar Heimstaedt created the first working fluorescent microscope in 1911.
- Highest Resolution Microscopy Techniques: Electron microscopy (EM) provides the highest resolution for cellular ultrastructure, capable of visualizing single atoms, while super-resolution light microscopy techniques like STED, PALM/STORM, and MINFLUX overcome the diffraction limit to offer significantly enhanced resolution for live biological samples.
- Top Applications of Biological Imaging: Biological imaging is crucial for drug discovery, studying disease pathology like cancer progression, and evaluating gene therapies, while also being extensively used to examine normal anatomy and physiology, enabling real-time observation of biological processes at various scales.
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